| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 41 | Clostridium neonatale | GGCCAAATGGAGCTGGTAAG | CCATCAGATGGCCTTTTAGCA | 58.89 | 58.34 | 223 | 138 |
100.00%
|
100.00%
|
| Primer 42 | Clostridium neonatale | TCCTTGGAGGACTTGGCAT | ACACGCCACAAATTCCAGG | 58.21 | 58.66 | 240 | 138 |
100.00%
|
100.00%
|
| Primer 43 | Clostridium neonatale | TTGCGGATGGAATGGGAGG | GCATGCTGTAACTGTCGTACC | 59.77 | 59.34 | 209 | 138 |
100.00%
|
100.00%
|
| Primer 44 | Clostridium neonatale | CCATCAATGGGAGTTGCACC | AACGCCTGTCTTTGTCCTCC | 59.18 | 60.25 | 206 | 138 |
100.00%
|
100.00%
|
| Primer 45 | Clostridium neonatale | CCATCAATGGGAGTTGCACC | CGCCTGTCTTTGTCCTCCTG | 59.18 | 60.67 | 204 | 138 |
100.00%
|
100.00%
|
| Primer 46 | Clostridium neonatale | CCATCAATGGGAGTTGCACC | TAACGCCTGTCTTTGTCCTCC | 59.18 | 60.00 | 207 | 138 |
100.00%
|
100.00%
|
| Primer 47 | Clostridium neonatale | GGAAAGTTCAGGGAAGGGCA | ATCATATCCCACTGCAACTGC | 59.89 | 58.42 | 238 | 138 |
100.00%
|
100.00%
|
| Primer 48 | Clostridium neonatale | TCAGGGAAGGGCAATAAAGCA | ATCATATCCCACTGCAACTGC | 59.64 | 58.42 | 231 | 138 |
100.00%
|
100.00%
|
| Primer 49 | Clostridium neonatale | CAGGGAAGGGCAATAAAGCA | ATCATATCCCACTGCAACTGC | 58.15 | 58.42 | 230 | 138 |
100.00%
|
100.00%
|
| Primer 50 | Clostridium neonatale | AGCTGGTGCAAAGGTCACA | GCCAATAAAAGGCCAGCTTTC | 59.77 | 58.38 | 172 | 137 |
100.00%
|
100.00%
|